Figure 5.

Fluorescence staining of infiltrated lymphocytes. Tumours were snap‐frozen, and 8‐μm sections were prepared in Tissue Tek (Sakura Finetek). Anti‐CD3⁺ (FITC conjugate, green) monoclonal antibody was used to detect lymphocytes infiltrated into tumours in situ. Tumours of mice treated with p‐c (D) showed more infiltrated lymphocytes than control groups treated with e‐p (C), liposome alone (B) or PBS (A). To further determine the type of infiltrated lymphocytes, anti‐CD3⁺ and anti‐CD8⁺ (Cy5PE conjugate, red) monoclonal antibodies were used simultaneously to stain the p‐c treated tumour tissue (D and E, respectively), and the merged image (F) confirmed that the infiltrated lymphocytes were mainly CD8⁺ CTLs.