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. 2008 Dec 16;13(8b):2158–2170. doi: 10.1111/j.1582-4934.2008.00616.x

Figure 1.

Figure 1

p53‐dependent activation of DUSP11. (A) Northern blot analysis for the expression of DUSP11, STRAIT11499, APAF1, EST1 and p21 (CDKN1A) mRNA isolated from U2OSp53ER cells. Cells were incubated with 4‐hydroxytamoxifen (OHT), cycloheximide (CHX) or both for the indicated times. UT: untreated cells. (B) qPCR analysis for the expression of p21 (CDKN1A) and DUSP11. U2OSp53ER cells were incubated with OHT, for the indicated times. UT: untreated cells. (C) Actinomycin D (ActD) treatment induces the expression of DUSP11, APAF1 and p21 (CDKN1A) mRNA, in a p53‐dependent manner. Upper part: Northern blot analysis of mRNA isolated from RKO and RKO + E6 cells. Cells were treated with 5 nM ActD for the indicated times. UT: untreated cells. The blots were probed for expression of DUSP11, APAF1 and p21 (CDKN1A). Total RNA is shown as a loading control. Lower part: Western blot analysis of cell lysates prepared from RKO cells or from RKO cells expressing E6. Cells were grown in the presence (+) or absence (−) of 5 nM ActD for 24 hrs before lysis. The blots were probed with anti‐p53, anti‐p21 and anti‐actin antibodies. (D) Doxorubicin treatment induces the expression of DUSP11, APAF1 and p21 (CDKN1A) mRNA, in a p53‐dependent manner. Upper part: Northern blot analysis of mRNA isolated from RKO and RKO + E6 cells. Cells were treated with 0.3 μg/ml Doxorubicin (Doxo) for the indicated times. UT: untreated cells. The blots were probed for expression of DUSP11, APAF1 and p21 (CDKN1A). Total RNA is shown as a loading control. Lower part: Western blot analysis of cell lysates prepared from RKO cells or from RKO cells expressing E6. Cells were grown in the presence (+) or absence (−) of 0.3 μg/ml Doxorubicin (Doxo) for 24 hrs before lysis. The blots were probed with anti‐p53, anti‐p21 and anti‐actin antibodies.