Figure 4.

Genotoxic stress induced binding of p53 to the first intron of DUSP11. (A) Schematic presentation of DUSP11 genomic region: the two putative p53‐binding sites, located in Intron 1, are indicated as A and B. (B) ChIP analysis of U2OS cells treated with 15 J/m₂ UV for the indicated times. UT: untreated cells. ChIP assays were performed using an antibody specific for p53 (DO1) and the anti‐Flag was used as a negative control. The resulting DNA was amplified by qPCR with primers corresponding to the genes shown. p21 (CDKN1A), HDM2 and PUMA promoters were used as positive controls, while Acetylcholine muscarinic receptor (AchR) promoter functioned as a negative control.