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. 2008 Dec 16;13(8b):2158–2170. doi: 10.1111/j.1582-4934.2008.00616.x

Figure 5.

Figure 5

Ectopic expression of DUSP11 inhibits cell proliferation. (A) U2OS cells were stably transfected with the indicated plasmids (pCMV: negative control, pCMV p21: control for the inhibition of cellular proliferation). The relative number of colonies is plotted in a graph (see ‘Materials and methods’). Error bars indicate standard deviations based on three independent transfections. (B) Western blot analysis showing the levels of overexpressed Dusp11 and C/S proteins. The blots were probed with anti‐HA antibody. (C) Mouse embryonic fibrolasts were infected with the indicated plasmids and selected in puromycin 2.5 μg/ml for 5 days. Once selected, they were plated in triplicate and their growth was analysed performing an MTT assay at the indicated time‐points. Error bars indicate standard deviations based on three independent experiments. (D) Western blot analysis of Dusp11, C/S and E2F1 overexpressed proteins at days 1, 3 and 6 after selection. The blots were probed with anti‐HA antibody.