Figure 1.

Time‐ and dose‐dependent induction of HO‐1 by TGF‐β₁ in HAoSMC. Confluent cultures were equilibrated in medium containing 1% FCS prior to treatment with TGF‐β₁ (0.1–10 ng/ml, 0–24 hrs). (A) Immunoblot analyses of HO‐1 protein expression in cells treated with TGF‐β₁ (2.5 ng/ml) relative to α‐tubulin used as a loading control and (B) denstometric quantification of HO‐1 expression. (C) Denstometric quantification of immunoblot analyses of HO‐1 expression in cells treated with TGF‐β₁ for 12 hrs. Immunoblots are representative of results obtained in n= 3–8 different cell cultures, data are means ± S.E.M., ★P < 0.05 relative to untreated cells.