Figure 2.

Time dependent adhesion of Caki‐1 to HUVEC. Caki‐1 cells were treated with low (0.25 mM) or high (1 mM) concentrations of VPA, applied alone or in combination with IFN‐α. After a three or five day pre‐incubation, tumour cells were added at a density of 0.5 × 10⁶ cells/well to HUVEC monolayers for different time periods. Non‐adherent tumour cells were washed off in each sample, the remaining cells were fixed and counted in five different fields (5 × 0.25 mm²) using a phase contrast microscope. Mean values were calculated from five counts. Mean adhesion capacity is depicted as counted cells/mm². * indicates significant difference to controls. # indicates significant differences between VPA monotherapy and VPA‐IFN‐α combination therapy. If VPA‐IFN‐α combination therapy was not superior to the VPA monotherapy but evoked significant differences to the untreated controls, respective figure symbols were also marked with *.