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. 2008 Nov 8;13(8b):1741–1750. doi: 10.1111/j.1582-4934.2008.00584.x

Figure 1.

Figure 1

Molecules of the CD40 signalling cascade are highly induced 48 hrs after TLR4 activation at the time of cytokine exhaustion. (A) Immature DCs were stimulated with LPS/IFN‐γ for 6 hrs and compared to un‐stimulated DCs by DNA micro‐array analysis. In addition, 12, 24 and 48 hrs matured DCs receiving a 6‐hr LPS/IFN‐γ stimulus were also compared to un‐stimulated DCs. Key molecules of the TLR and CD40 signalling cascade are analysed by heat plotting over the time. Up‐regulation is indicated by red and down‐regulation by different green colouring. (B) Detailed illustration of differentially regulated genes encoding secreted proteins or proteins involved in TLR or CD40/CD40L signalling. mRNA fold expression is depicted over 48 hrs after LPS/IFN‐γ activation at a linear scale. *Down‐regulation compared to immature DCs. (C) Quality control by analysing the activation status of LPS/IFN‐γ‐treated DCs in (A). IL‐10, IL‐12 and the IDO metabolite kynurenine were measured as depicted over 48 hrs of maturation. These data were reproducible using two different donors. (D) Expression density of TLR4, CD14 and CD40 after 48 hrs of LPS/IFN‐γ activation (black histogram) compared to un‐stimulated immature DCs (dashed histogram) and isotype control stained immature DCs (filled black histogram). One out of two independent experiments using two different donors is depicted.