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. 2019 May 22;25:22. doi: 10.1186/s10020-019-0091-4

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of EA stimulus on the expression of α7nAChR. a Compared with sham group, the cerebral I/R injury decreased the contents of α7nAChR. Meanwhile, the EA up-regulated the contents of α7nAChR compared with MCAO group. *P < 0.05 vs. Sham group, ^##P < 0.01 vs. MCAO group. b Representative immunofluorescent staining of α7nAChR, neuronal nuclei (NeuN), and nuclear marker DAPI. Cerebral I/R injury decreased the levels of α7nAChR-positive neurons compared with Sham group, while EA up-regulated the contents of α7nAChR-positive neurons when compared with MCAO group. Scale bar = 30 μm