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. 2019 May 22;21:67. doi: 10.1186/s13058-019-1153-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Knockdown of leptin expression in obASCs decreases promotion of metastatic phenotype. a Western blots of signaling pathways activated in BCCS after Transwell co-culture with control shRNA obASCs compared to leptin shRNA obASCs. b RT-qPCR after transwell co-culture with obASC or obASC with leptin shRNA demonstrates decreased mRNA levels of Serpine1, TWIST1, and SNAI2. c Leptin shRNA obASCs decreased migration of BT20, HCC1806, and MCF7 breast cancer cell lines through a 0.4-um transwell membrane. Values reported are the mean of three independent experiments each performed in triplicate. Bars, ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001