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Elevated level of oxidative stress in young and old mouse muscles. (a) Representative western blots of protein extracted from young and old muscle samples. Densitometry quantification of (b) SOD2, (c) NOX2, and (d) GPx1. Values are normalized to the expression levels of the housekeeping gene GAPDH. Results are means ± SD (n = 5-6, ∗p < 0.05, #p < 0.01); data were analyzed using an unpaired t-test.
