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. 2019 May 7;2019:4651627. doi: 10.1155/2019/4651627

Figure 4.

Figure 4

Hydrolytic activity of CD39 and CD73 solubilized in cervical samples from CIN-1 patients and NDs. Aliquots of 2 μg of total protein obtained from the supernatants of cervical samples from NDs negative for HPV (ND, black bars) and CIN-1 patients, positive either for HPV-16 (HPV-16, white bars) or for coinfection with HPV-16 and other HPV types (HPV-16+CI, diagonal lines), or HPV patients negative for HPV-16 (HPV-16neg, horizontal lines) were incubated in the presence of 5 mM of ATP or AMP and in the presence or absence of POM-I or APCP, specific inhibitors of CD39 and CD73, respectively. After 72 h of incubation, the Ado product of ATP (a) or AMP (b) hydrolysis was evaluated by UPLC. ∗ indicates a significant difference (P < 0.01) of the Ado product of CIN-1 samples compared to NDs. Data represent three independent experiments.