Fig. 6.

Umbelliferone production with different 4CLs and Pc4CL1 with different mutants. (a), The yield of umbelliferone in different strains expressing different 4CLs was measured for 60 h at 12 h increments. (b), The yield of umbelliferone with different Pc4CL1 mutations was measured at 48 h. V, Q, F, Y, VQ, QF, QY, VQF, VQY denotes Pc4CL1 V184G, Q272H, F267 L, Y240A, V184G/Q272H, Q272H/F267 L, Q272H/Y240A, V184G/Q272H/F267 L, V184G/Q272H/ Y240A mutations, respectively. All data are represented as mean ± SD from three independent experiments (n = 3). Error bars are defined as SD