Table 3.
Strains and plasmids used and constructed in this study
| Description | Source | |
|---|---|---|
| Strains | ||
| E.coli DH5a | General cloning host | Invitrogen |
| E. coli K12 (MG1655) | F-, λ-, ilvG-, rfb-50, rph-1 | Novagen |
| E.coli BL21(DE3) | F-, ompT, hsdS (rB− mB−), gal, dcm (DE3) | Novagen |
| K12 | E. coli K12 carrying the gene for T7 RNA polymerase in pGEX6P-1 | This study |
| P | K12ΔpheA | This study |
| E | K12ΔtrpE | This study |
| PE | K12ΔpheAΔtrpE | This study |
| PET | K12ΔpheAΔtrpEΔtyrR | This study |
| PET-pRSF | PET carrying pRSF | This study |
| K12-pCDF | K12 carrying pCDF | This study |
| P-pCDF | P carrying pCDF | This study |
| E-pCDF | E carrying pCDF | This study |
| PE-pCDF | PE carrying pCDF | This study |
| PET-pCDF | PET carrying pCDF | This study |
| PET-pRSF-pCDF | PET-pRSF carrying pCDF | This study |
| PET-pRSF-Pc4CL1 | PET-pRSF carrying pCDF-Pc4CL1 | This study |
| PET-pRSF-Pt4CL | PET-pRSF carrying pCDF-Pt4CL | This study |
| PET-pRSF-At4CL1 | PET-pRSF carrying pCDF-At4CL1 | This study |
| PET-pRSF-At4CL2 | PET-pRSF carrying pCDF-At4CL2 | This study |
| PET-pRSF-Pc4CL1-M | PET-pRSF carrying pCDF-Pc4CL1 (Q272H/F267 L) | This study |
| Plasmids | ||
| pET28a | T7 promoters, pBR322 ori, KnR | Novagen |
| pRSFDuet-1 | Double T7 promoters, RSF ori, KnR | Novagen |
| pCDFDuet-1 | Double T7 promoters, CloDF13 ori, SmR | Novagen |
| pRSF-1 | pRSFDuet-1 carrying tktA | This study |
| pRSF-2 | pRSFDuet-1 carrying tktA, aroB, aroE and aroK | This study |
| pRSF | pRSFDuet-1 carrying tktA, aroGfbr, tyrAfbr, aroB, aroE and aroK | This study |
| pCDF-1 | pCDFDuet-1 carrying RgTAL | This study |
| pCDF-2 | pCDFDuet-1 carrying RgTAL, Ad4CL | This study |
| pCDF | pCDFDuet-1 carrying RgTAL, Ad4CL, AdC2’H | This study |
| pCDF-Pc4CL1 | pCDFDuet-1 carrying RgTAL, Pc4CL1, AdC2’H | This study |
| pCDF-Pt4CL | pCDFDuet-1 carrying RgTAL, Pt4CL, AdC2’H | This study |
| pCDF-At4CL1 | pCDFDuet-1 carrying RgTAL, At4CL1, AdC2’H | This study |
| pCDF-At4CL2 | pCDFDuet-1 carrying RgTAL, At4CL2, AdC2’H | This study |