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. 2019 May 7;2019:6581217. doi: 10.1155/2019/6581217

Figure 5.

Figure 5

The effects of miR-146a-5p inhibition in H9c2 cells under IH condition are reversed by XIAP knockdown. miR-146a-5p inhibitor, corresponding scrambled control, and small interfering RNA targeting XIAP (si-XIAP) were transfected into H9c2 cells. Cells without transfection were acted as control. (a–f) Cell apoptosis by flow cytometry analysis. (g) Cell viability by a Cell Counting Kit-8. (h, i) Western blotting assays for XIAP, Bcl-2, Bax, and Caspase-3 protein expressions. Relative protein levels are presented as the average expressions normalized to actin. IH: intermittent hypoxia; n = 3. (Data are presented as the mean ± SD of three independent experiments. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001).