Table 2.
Sequence type, antibiotic genetic determinant, and phylo-group of fecal and water isolates that had blactx-M or blaCMY -2, blatem or blashv genes.
| Strain | Round | ST | ESBL phenotype | Resistance Phenotype | AR Genes | Phylo-grou |
|---|---|---|---|---|---|---|
| Fecal | ||||||
| F4.1 | Rl | ST 2614 | ND | AMP-AMC-CTX | blatem | B2 |
| F7.1 | Rl | ST5914 | Negative | AMP-AMC-CF-N | blatem | Bl |
| F2.7 | Rl | ST8371 | Negative | AMP-AMC | blatem | D |
| F11.l | R2 | ST131 | CAZ,CTX,CRO, FOX, CF | AMP-CTX-CF-XNL-S-SXT-T | blactx, strA, sul1, tet (A), tet(M) | B2 |
| F11.2 | R2 | ST131 | CAZ,CTX,CRO, FOX, CF | AMP-CAZ-CF-XNL-S-SXT-T | blactx, strA, str B, sul1, tet(A), tet (B), tet (M) | B2 |
| F13.1 | R2 | ST131 | CAZ,CTX,CRO, FOX, CF | AMP-CAZ-CF-XNL-NA-S-SPT-SXT-T | blactx, str A, str B, sul1, tet(A) | B2 |
| F13.2 | R2 | ST131 | CAZ,CTX,CRO, FOX, CF | AMP-CAZ-CF-XNL-S-SPT-SXT-T | blactx, str A, str B, sul1, tet(A), tet (M) | B2 |
| F14.1∗ | R2 | ST 58 | ND | No resistance | No resistance | Bl |
| F15.2 | R2 | ST131 | CAZ,CTX,CRO, FOX, CF | AMP-CAZ-XNL-N–S-SXT-SPT-T | blactx-M, str A, str B, sul1, tet (A), tet (M), tetB | B2 |
| F16.2 | R2 | ST131 | CAZ,CTX,CRO, FOX, CF | AMP-CAZ-CF-XNL-NA-SXT-T | blactx-M, blaCMY -2, str A, str B, sul1, tet(A), | B2 |
| F20.3∗∗ | R2 | ST68 | CAZ,CTX,CRO, FOX,CF | AMP-AMC-CAZ-XNL-N-S-T | blactx, blaCMY -2, blashv, str A, str B, tet(A) | F |
| F27.2∗∗ | R2 | ST68 | CAZ,CTX,CRO, FOX, CF | AMP-AMC-CAZ-XNL-T | blactx, blaCMY -2, blatem, tet (A) tet (M) | F |
| F31.1 | R2 | unknown | Negative | AMP-AMC-CTX-XNL- | blaCMY -2_ strB, tet(B), | B2 |
| F32.1 | R2 | ST10 | Negative | AMP-CAZ-XNL-C-NA-S-T | blaCMY -2, str A, tet (A) | A |
| F34.2 | R3 | ST7348 | Negative | AMP-AMC-XNL-C | BlaCMY -2 tet (M) | Bl |
| F35.2 | R3 | ST2541 | CAZ,CTX,CRO | AMP-CAZ | blactx | A |
| F 42.2 | R4 | Unknown | Negative | AMP-AMC-CAZ-XNL-NA-N-S | blaCMY -2 strB | Bl |
| F43.1 | R4 | ST7207 | Negative | AMP-AMC-XNL-C-NA-N-S-T | blaCMY -2 strB, tet(B), tet(M) | A |
| F44.2 | R4 | Unknown | Negative | AMP-CAZ-XNL-NA-N | blaCMY | E |
| F46.2 | R4 | ST357 | Negative | AMP-CAZ-NA-N-S-T | blaCMY -2 strB, tet(B) | B2 |
| F47.2 | R4 | ST58 | Negative | AMP-AMC-XNL-ENO-NA-N-S | blaCMY -2, blatem, str A | Bl |
| F48.1 | R4 | ST3727 | Negative | AMP-AMC-XNL-NA-N | blaCMY -2 | C |
| F52.2 | R5 | ST195 | Negative | AMP-AMC-CAZ-C | blaCMY -2 | C |
| Water | ||||||
| NC5.1ctx | R2 | ST131 | CAZ,CTX,CRO, FOX | AMP-CAZ-XNL-S-SXT–T | blactx, str B, sul1, tet(A), tet (M) | B2 |
| SW2.3 ctx | R2 | ST1625 | Negative | AMP-AMC-CTX-C-AZ-XNL | blaCMY -2 | Bl |
| NC5.3 | R3 | ST2721 | Negative | A-AMC-XNL-CF-S-T | blaCMY -2 str B, tet (A) | Cryptic Clade III,IV,V |
| NC6.2∗ | R3 | ST58 | ND | No resistance | No resistance | Bl |
| NC 6.7 | R3 | ST10 | Negative | AMP-CAZ-XNL-C-NA-S-T | blaCMY -2blashv, strB, tet(B) | A |
| SW2.4 | R3 | ST83 | Negative | AMP-AMC-CAZ-XNL-NA-SPT-S-T | blacmy-2strB | B2 |
| NC5.2 | R3 | ST1204 | Negative | AMP-AMC-XNL-CF-S-T | blaCMY -2 blashv, str A,strB, tet (M) | D |
| RP3.1ctx | R3 | Unknown | CAZ-CTX-CRO, FOX | AMP-CTX-XNL-NA-SPT-S-SXT-T | blactx, aadA,sull, tet(M) | E |
| RP3.1 (Box) | R3 | ST5463 | Negative | AMP-CTX-XNL-CF-NA-SPT-S-SXT-T | blashv, str A, sul1, tet (B) | E |
| RP3.2∗ | R4 | ST58 | Negative | AMP-AMC-CTX-XNL-ENO-NA-N | blaCMY -2 | Bl |
| RP3.5 (CAT) | R4 | ST469 | Negative | AMP-XNL-CF-NA-S | blatem, strA | Bl |
| SW2.2 | R4 | ST1065 | Negative | AMC-CTX-CF-S | bla, strA,strB | B2 |
| SW2.4 | R5 | ST1850 | Negative | AMP-AMC-CAZ-CF-XNL-S-SXT-C | blaCMY.2 strB, sull | A |
| RP3.6 | R5 | Unknown | Negative | CF-XNL | blaCMY | C |
| RSI.3 | 6/16/2017 | Unknown | CAZ-CTX-CRO-CF | AMP-CAZ-CF-XNL-S-SXT-C | blactx, tet(B), str A sul1 | E |
| RS2.1 Mcoli | 4/6/2018 | ST297 | CAZ-CTX-CRO-FOX-CF | AMP-AMC-CAZ-CF-CRO | blactx | B1 |
∗Selected because they had same mdh (282 bp) sequence (Ivanetich et al., 2006). AR, antibiotic resistance; ST, Sequence Type. R1, R2, R3, R4, R5 are collection dates from 8/20/14, 9/17/14, 1/21/15, 2/27/15, 4/5/15, respectively. RS2.1 Mcoli was an isolate with the bla ctx gene that was found in the summer of 2018 (June–August) from two rounds of sampling that screened 15 isolates. ∗∗Indicate isolates that had both bla ctx-M and bla cmy-2 genes.