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. 2019 May 15;10:1021. doi: 10.3389/fimmu.2019.01021

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Copyright © 2019 Hutchinson, Mesa, Alexander, Yu, O'Rourke, Limoli, Wrin, Deeks and Berman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic analysis of proviral sequences isolated from an elite suppressor (EN3) and a normal progressor (EN1) that both possess bNAbs. Proviral sequences were recovered from two individuals (EN1 and EN3) using the Monogram Phenosene® assay system. Evolutionary analyses were conducted in MEGA6 using a Maximum Likelihood method with 1000 bootstrap replicates and a discrete Gamma distribution to model evolutionary rate. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. Sequences used in mutagenesis studies, EN3d071 and EN3c059, are shown with a black rectangle.