Table 4.
Infectivity of wild type and mutant EN3d071 pseudoviruses.
| Rank to relative light units (RLU) Key | |||||
|---|---|---|---|---|---|
| Proviral clones | Number of Cys in gp160 | Tropism | R5 infectivity (Rank) | X4 infectivity (Rank) | 0 Rank < 5,000 RLU (no infectivity detected) |
| 0.5 Rank < 5,000 RLU (infectivity detected) | |||||
| EN3d071 WT | 22 | R5 | 2 | 0 | 1 Rank = 5,001 to 15,000 RLU |
| EN3d071 R(134+1)C* | 23 | N/A | 0 | 0 | 2 Rank = 15,001 to 150,000 RLU |
| EN3d071 C136A | 21 | R5 | 2 | 0 | 3 Rank = 150,001 to 1,000,000 RLU |
| EN3d071 K170E | 22 | R5 | 0.5 | 0 | 4 Rank = >1,000,000 RLU |
Viral tropism was determined using the Trofile® DNA assay system from Monogram Biosciences. CD4+/U87 cells expressing CXCR4 or CCR5 coreceptor were inoculated with pseudoviruses. Infected cells produced luciferase. Tropism was determined by the relative light units (RLU) in lysed cells 72 h after inoculation.
*
Infectivity too low for use in the neutralization assay.