Table 1.
Trial 1 | Trial 2 | Trial 3 | |
---|---|---|---|
Sample types | Water, Swabs, Oysters | Water, Swabs, Oysters | Water |
Sampling days (0 = spawn) | W:1,12 / OS:5,12 | W:1,9 / OS:6,9 | W: 5,8,12 |
Volume water filtered | 410–750 mL | 7–10 mL | 1300–1700 mL |
Trial dates | July 11–23, 2012 | Jan 9–18, 2013 | June 3–15, 2016 |
Bacterial reads from 12 water samples | 1.3 million | 1.8 million | 5.7 million |
Methods | Mo Bio extraction MiSeq, 2x250 PE | Mo Bio extraction MiSeq, 2x250 PE | Puregene extraction HiSeq, 2x100 PE |
16S region | V4 (515F/806R) | V4 (515F/806R) | V6 (967F/1064R) |
W, water; S, swab; O, oysters.