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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Biochem Pharmacol. 2018 Oct 4;158:103–113. doi: 10.1016/j.bcp.2018.10.003

Figure 1:

Figure 1:

Concentration-dependent effect of reference A3AR agonist 2-Cl-IB-MECA on βarr2 recruitment in a stable hA3AR-NanoBit®-βarr2 HEK293T cell line. 1, 2, 5, 10, 30, 50, 80, 100, 500 nM and 1 µM 2-Cl-IB-MECA was added at the time point indicated by the arrow, and luminescence was measured for >90 min. A solvent control of 0.01% DMSO was included. Insert: Concentration-response (AUCs). AUCs (± SEM) of quadruplicate wells are shown for a representative experiment (n=3).