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. Author manuscript; available in PMC: 2019 May 22.
Published in final edited form as: Ann Rheum Dis. 2010 Jun 22;69(11):2017–2023. doi: 10.1136/ard.2009.127761

Figure 3.

Figure 3

Steady state mRNA levels for types I and III collagens and fibronectin (FBN) in normal and nephrogenic systemic fibrosis (NSF) fibroblasts. (A) Northern hybridisations of total RNA from cultured NSF and normal (N) fibroblasts. (B–D) Densitometric assessment of the intensity of mRNA bands in the northern blots shown in (A). Pixel density was measured by Image J software. Each band was quantified and divided by the corresponding housekeeping band density value. The ratio was set to 1 for the first control sample and all others values were calculated as a multiple thereof. GAPDH, glyceraldehyde phosphate dehydrogenase.