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. Author manuscript; available in PMC: 2019 May 22.
Published in final edited form as: J Am Chem Soc. 2016 Nov 8;138(45):14931–14940. doi: 10.1021/jacs.6b07584

Figure 4:

Figure 4:

Correlative microscopy flow-chart. Step1: Cells were grown on gold finder grids with fiducial markers that allow navigation to desired locations on the grid. The cells were incubated for 48 h with acLDL. Step2: Cells were fixed (4 % PFA, 0.1% GTA, see methods) and incubated with primary and secondary antibodies. Step 3: super-resolution fluorescence signal was resolved by STORM. Step4: Grids were high pressure frozen. Step5: X-ray tomograms were taken of the same cells that were analyzed by STORM. Data were reconstructed (field of view 15 μm3). Step6: Overlay of the data was done manually using the grid mesh as fiducial markers for the xy plane. For the z plane, each of the X-ray and fluorescence volume stacks was divided into layers of 80 nm and overlaid (see experimental section).