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. 2019 May;60(6):2274–2285. doi: 10.1167/iovs.18-26077

Figure 1.

Figure 1

Phosphorylation of cofilin in porcine retinal explants increases after detachment over 24 hours while total cofilin remains stable. (A) Cofilin levels were examined within one animal: retinal explants incubated for 0, 1, 5 10, 30 minutes from one eye, 0, 1, 3, 7, 24 hours for the other eye. Antibodies against p-cofilin and t-cofilin labeled 19-kDa bands. GAPDH, from the same SDS-PAGE gel, served as an internal loading control. (B) Quantification of cofilin bands, normalized with GAPDH. n = 3 animals, 30 retinal explants; 1-way ANOVA compared within groups 0 to 30 minutes and 0 to 24 hours, *P < 0.05, **P < 0.01, ***P < 0.001; post hoc Tukey's test, a: 1 vs. 24 hours, <0.01, b: 3 vs. 24 hours, <0.05; b: 7 vs. 24 hours, <0.05.