Skip to main content
. 2019 Apr 19;160(6):1439–1447. doi: 10.1210/en.2019-00132

Figure 4.

Figure 4.

Suppression of IGF-1R activity induces GHR expression and increases DNA damage in human 3D intestinal organoids. (A) Representative Western blot of organoids treated with 100, 300, and 600 nM PPP and harvested 24 h later. (B) ImageJ quantification of protein expression in (A) normalized to loading control. Results shown are mean ± SEM of three independent experiments. (C) Olive tail moment in organoids treated with 500 ng/mL GH (GH), 300 nM PPP (PPP), or 300 nM PPP and 500 ng/mL GH (PPP + GH) and harvested 24 h later. Results shown are mean ± SEM of three independent experiments. Differences were assessed with Tukey-adjusted mixed model regression. *P < 0.05, **P < 0.01 vs control. Data are graphed as percentage of control, and statistical testing was performed on individual values.