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. 2019 May 15;10:551. doi: 10.3389/fphys.2019.00551

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Copyright © 2019 Lou, Shen, Li, Huang, Lu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of RNAi on oviposition in dsGFP (control) and dsNlESMuc groups. (a,b) The eggs laid in rice seedlings; the upper right corner shows an enlarged image. Arrowheads indicate the eggs laid in both groups. The dsNlESMuc eggs were partially inside the rice tissue, leaving the anterior half and micropyle area exposed. (c) The average number of eggs laid per female in both groups. The dsNlESMuc females had significantly reduced fecundity. (d) The hatchability in both groups. The hatchability in dsNlESMuc group was significantly affected when compared to that in dsGFP group. (e) The duration from ovipositor penetration to withdrawal. The process was longer after NlESMuc knockdown. (f) RNAi efficiency. Not significant (ns) P ≥ 0.05, ^∗∗P ≤ 0.001.