Fig. 5. ESI MS analysis of the PDZ–peptide covalent conjugates. (A) The proteins were incubated with peptides equipped with different linkers (protein/peptide 15/75 μM, pH 7.4, 12 hours). (B) The mass analysis of peptide–PDZΔRGS3 conjugation, figures prepared using Prism based on original MS data shown in Fig. S14–S18.† (C) Mass/mass spectrometry analysis of trypsin-digested PDZ–peptide conjugates indicating that peptide PD3-I binds covalently to C33 or C34 in PDZΔRGS3.
