Table 2.

Primers used in this study.

Primer	Sequence (5′–3′)	Target fragment length (bp)	Annealing temperature (°C)	References
HblA-F	GTGCAGATGTTGATGCCGAT	320	55	Hansen and Hendriksen, 2001
HblA-R	ATGCCACTGCGTGGACATAT
HblC-F	AATGGTCATCGGAACTCTAT	750	55	Hansen and Hendriksen, 2001
HblC-R	CTCGCTGTTCTGCTGTTAAT
HblD-F	AATCAAGAGCTGTCACGAAT	430	55	Hansen and Hendriksen, 2001
HblD-R	CACCAATTGACCATGCTAAT
NheA-F	TACGCTAAGGAGGGGCA	500	55	Hansen and Hendriksen, 2001
NheA-R	GTTTTTATTGCTTCATCGGCT
NheB-F	CTATCAGCACTTATGGCAG	770	55	Hansen and Hendriksen, 2001
NheB-R	ACTCCTAGCGGTGTTCC
NheC-F	CGGTAGTGATTGCTGGG	583	55	Hansen and Hendriksen, 2001
NheC-R	CAGCATTCGTACTTGCCAA
cytK-F	AAAATGTTTAGCATTATCCGCTGT	238	55	Oltuszak-Walczak and Walczak, 2013
cytK-R	ACCAGTTGTATTAATAACGGCAATC
cesB-F	GGTGACACATTATCATATAAGGTG	1271	58	Ehling-Schulz et al., 2005
cesB-R	GTAAGCGAACCTGTCTGTAACAACA
glpF-F	GCGTTTGTGCTGGTGTAAGT	549	59	PubMLST (http://pubmlst.org/bcereus/info/primers.shtml)
glpF-R	CTG CAATCGGAAGGAAGAAG
gmk-F	TTAAGTGAGGAAGGGTAGG	600	56
gmk-R	AATGTTCACCAACCACAA
ilvD-F	GGGCAAACATTAAGAGAA	556	58
ilvD-R	TTCTGGTCGTTTCCATTC
pta-F	AGAGCGTTTAGCAAAAGAA	576	56
pta-R	CAATGCGAGTTGCTTCTA
pur-F	GCTGCGAAAAATCACAAA	536	56
pur-R	CACGATTCGCTGCAATAA
pycA-F	GTTAGGTGGAAACGAAAG	550	57
pycA-R	CGTCCAAGTTTATGGAAT
tpi-F	CCAGTAGCACTTAGCGAC	553	58
tpi-R	GAAACCGTCAAGAATGAT