Table 1.
List of primer sequences used for qRT-PCR analysis
| Gene name | Primer sequences (5′ to 3′)a |
|---|---|
| IGFBP3 | IGFBP3 F: ATGGTCCCTGCCGTAGA |
| IGFBP3 R: CCCGCTTCCTGCCTTT | |
| E-cadherin | E-cadherin F: GAACGCATTGCCACATAC |
| E-cadherin R: ACCTTCCATGACAGACCC | |
| N-cadherin | N-cadherin F: TCCTGCTTATCCTTGTGC |
| N-cadherin R: GTCCTGGTCTTCTTCTCCT | |
| Smad1 | Smad1 F: ATGCCACTCAACGCCACTTT |
| Smad1 R: CCGCCTGAACATCTCCTCTG | |
| Smad2 | Smad2 F: AATGCCACGGTAGAAATGAC |
| Smad2 R: ACAGACTGAGCCAGAAGAGC | |
| Smad3 | Smad3 F: GGAGGAGAAATGGTGCGAGAA |
| Smad3 R: CCACAGGCGGCAGTAGAT | |
| Smad4 | Smad4 F: GGATACGTGGACCCTTCT |
| Smad4 R: CGATGACACTGACGCAAA | |
| Smad5 | Smad5 F: TAGCAGCACATATCCCAACT |
| Smad5 R: AGGCTCTTCATAGGCAACA | |
| Smad6 | Smad6 F: CCCCATCTTCGTCAACTCC |
| Smad6 R: GCTGATGCGGACGCTGTT | |
| Smad7 | Smad7 F: AACCGCAGCAGTTACCC |
| Smad7 R: CGAAAGCCTTGATGGAGA | |
| Smad8 | Smad8 F: ACATTCCAGGCTTCCTCCCG |
| Smad8 R: TGCTGTCACTCACGCACTCG | |
| β-actin | β-actin-F: GTGGACATCCGCAAAGA |
| β-actin-R: CTCGTCATACTCCTGCTTG |
a
F, forward; R, reverse