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. Author manuscript; available in PMC: 2019 May 22.
Published in final edited form as: Science. 2016 Jun 17;352(6292):1474–1477. doi: 10.1126/science.aad5168

Fig. 3. NAD+ fluctuations in cells.

Fig. 3

(A) Free NAD+ concentrations in HEK293T cells following treatment with FK866 (10 nM). Fluorescence of Sensor/cpVenus (488/405 nm) were measured by flow cytometry and the fold- change compared to untreated controls was interpolated onto an in vitro standard curve. (B) Cytoplasmic free NAD+ was decreased by NAMPT depletion and partially restored by NR treatment (100 µM). Imaging measurements from ex. 488 nm. REML, mean±SEM, n=3, ***p<0.001, *p<0.05. (C) Effect of free NAD+ availability on PARP activation.