Figure 6. Efficient delivery of DNA and mRNA by the polyplexes in multiple cell lines.
(A) and (B): Transfection efficiency of the (A) DNA and (B) mRNA polyplexes in HCT116 cells. (C) and (D): Transfection efficiency of the (C) DNA and (D) mRNA polyplexes in NHDF cells. The weight ratios of PBAP:PEG–PBAP–PEG:mRNA or CLPBAP:PEG–CLPBAP–PEG:mRNA in the PBAP and CLPBAP polyplexes, respectively,were fixed at 48:24:1. The AD:β-CD molar ratio in the crosslinked PBAP polyplexes was fixed as 4:3*: p < 0.05; **: p < 0.01; ****: p < 0.0001; n = 3.