Skip to main content
. Author manuscript; available in PMC: 2019 May 22.
Published in final edited form as: ACS Appl Mater Interfaces. 2018 Sep 17;10(38):31915–31927. doi: 10.1021/acsami.8b09642

Figure 7. Efficient delivery of CRISPR-Cas9 gene editing machinery by polyplexes.

Figure 7

(A) Genome editing efficiency of non-crosslinked and crosslinked RNP polyplexes in mCherry-expressing HEK 293 cells. The RNP polyplexes were prepared by varying the polymer-to-RNP weight ratios (i.e., PBAP: PEG–PBAP–PEG: RNP for non-crosslinked polyplexes, or CLPBAP: PEG–CLPBAP–PEG: RNP for crosslinked polyplexes). mCherry knock-out efficiency was assayed by flow cytometry for loss of mCherry fluorescence. (B) Precise gene correction efficiency of the non-crosslinked and crosslinked S1mplex polyplexes in BFP-expressing HEK 293 cells. S1mplex polyplexes were prepared by varying the polymer-to-S1mplex weight ratios (i.e., PBAP: PEG–PBAP–PEG: S1mplex for non-crosslinked polyplexes, or CLPBAP: PEG–CLPBAP–PEG: S1mplex for crosslinked polyplexes). Precise gene correction efficiency of the BFP to the GFP from the S1mplex repair ssODN repair template was assayed by flow cytometry for gain of GFP fluorescence. NS: not significant; n = 3.