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Cell viability study of the non-crosslinked and crosslinked complexes loaded with (A) DNA, (B) mRNA, (C) RNP, and (D) S1mplex. Cytotoxicity was studied using the polymer/payload formulation yielding the highest transfection efficiency for DNA or RNA payloads or genome editing efficiency for RNP or S1mplex payloads. NS: not significant; *: p < 0.05; **: p < 0.01; ***: p < 0.005; n = 5.
