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Heparin displacement analysis of A) high-H3 AuNP complexes; B) mid-H3 AuNP complexes; C) low-H3 AuNP complexes; D) K5 AuNP complexes; and E) PEI polyplexes, all at an N:P ratio of 4. The AuNP-pDNA complexes and PEI polyplexes were incubated in the presence of increasing amounts of heparin for 0.5 h at 37 °C and the incubated samples were subsequently analyzed by agarose gel electrophoresis.
