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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Curr Protoc Neurosci. 2019 Mar 18;87(1):e66. doi: 10.1002/cpns.66

Figure 4. Transduction of primary rat cortical neurons with a concentrated lentivirus.

Figure 4

Lentiviral transfer vector FCK-ChR2-GFP delivering Channelrhodopsin-2 tethered to eGFP expressed from a cytomegalovirus (CMV) promoter was prepared as described in Protocol 2. The preparation had a yield of 1.2e8 TU/ml. 5 ul of virus was used to infect 1e6 cultured mouse primary neurons. eGFP expression was observed weakly at 24 hours and robustly after 48 hours.