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Time Considerations for AAV
| Process | Time |
|---|---|
| Transfection of cells with recombinant viral genome to collection of unconcentrated virus | 8 days |
| Ultracentrifugation and small-scale purification of virus | 2 days |
| Ultracentrifugation and large-scale purification of virus | 2 days |
| Titer by qPCR or ddPCR and silver stained gels | 1 day |
| In vitro and ex vivo titering method | 3 days to 2 weeks |
