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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Curr Protoc Neurosci. 2019 Mar 18;87(1):e66. doi: 10.1002/cpns.66

Table 5.

Time Considerations for Retroviruses and Lentiviruses

Process Time
Transfection of cells with recombinant viral genome to collection of unconcentrated virus 4 days
Ultracentrifugation to concentrate virus 1 day
Titer by qPCR or flow cytometry 6 days