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. 2018 Mar 12;24(6):318–326. doi: 10.1093/molehr/gay014

Figure 1.

Figure 1

Real-time PCR and immunofluorescence for RAGE in cumulus granulosa cells treated with media alone or media with 1,25 dihydroxyvitamin D3 (vit D3). Pooled cumulus granulosa cells of women undergoing oocyte retrieval following ovarian stimulation for IVF were mechanically collected. Cells were treated with media alone or media + vit D3 (100 nM) for 24 h after which (A) RT-PCR for RAGE mRNA (n = 4) and (B) immunofluorescence for RAGE protein (n = 4) were performed. The addition of vit D3 significantly suppressed RAGE mRNA and protein levels. For immunofluorescence, the positive signal is seen in green and the blue signal represents DAPI; (a, b) are for cells treated with media alone (control) and (c, d) are for cells treated with media + vit D3. *P < 0.05 for media + vit D3 versus media alone.