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. 2018 Mar 12;24(6):318–326. doi: 10.1093/molehr/gay014

Figure 2.

Figure 2

Real-time PCR for steroid gene expression following treatment of cumulus granulosa cells with human glycated albumin (HGA), as a precursor for AGEs, with or without 1,25 dihydroxyvitamin D3 (vit D3). Pooled cumulus granulosa cells of women (n = 4) undergoing oocyte retrieval following ovarian stimulation for IVF were mechanically collected by cutting the cumulus layer from each oocyte. Cells were treated with HGA (0.4 mg/mL) with or without vit D3 (100 nM) for 24 h after which real-time PCR was performed. HGA significantly increased CYP11A1 (side chain cleavage), 3β-HSD, StAR, CYP17A1 and LHR mRNA expression levels but did not alter CYP19A1 or FSHR mRNA levels. The addition of vit D3 to HGA inhibited the HGA-induced increase in CYP11A1, StAR, CYP17A1 and LHR but not increase in 3-βHSD mRNA levels. The combination of HGA + vit D3 significantly increased CYP19A1 mRNA expression levels. Controls cells were set at 1. *P < 0.05 for HGA versus control, and *P < 0.05 for HGA + vit D3 versus control.