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. 2019 May 22;14(5):e0217030. doi: 10.1371/journal.pone.0217030

Fig 6. Photomicrographs of immunohistochemical staining (brown) of RAS in rat submandibular gland.

Fig 6

Targets: REN, ACE, ACE-2, AT1, AT2 and MAS were analyzed. Arrow colors indicate as follows: blue = myoepithelial cell; black = duct cells; red = blood vessels; yellow: acinar cells. GCT = granular convoluted tubule. No REN expression was observed in submandibular glands under all treatments. ACE shows higher expression in blood vessels (red arrowhead) under all treatments. ACE expression in duct cells (black arrowhead) and myoepithelial cells around intercalated ducts (blue arrowhead) and acini (yellow arrowhead) are higher in saline treatment than those in rat glands treated with losartan and isoproterenol. ACE-2 is present in acinar cells (yellow arrowhead) and the duct system (black arrowhead) except in the granular convoluted tubule cells (GCT). Mild ACE-2 expression in acinar cells can be observed in rat glands treated with isoproterenol. AT1 and AT2 are present in acinar cells (yellow arrowhead) and striated/excretory ducts (black arrow head), but only AT1 is expressed in GCT. AT1 is weakly expressed in rat glands treated with losartan and a strong immunostain for MAS is observed only in the GCT. (x40 objective and scale bar = 50 μm).