Fig 7. RNA-protein interaction detection (RAPID) approach to identify proteins that associate with C3U viral RNA in living cells.

(A) Schematic overview of RAPID. Viral and miR-122 RNAs are depicted. Putative binding protein complexes are shown as ovals. See text for details. (B) Abundances of HCV core and cellular actin proteins in cell extracts isolated from non-transfected (NT) and viral RNA-transfected cells. A Western blot is shown. (C) List of biotinylated proteins that interact uniquely with the C3U-BoxB viral genome. Complete list of identified proteins can be found the Excel-File in the Appendix. (D) Kinetics of wildtype (WT) and mutant C3U (C3U) RNA abundances in RNA- transfected Huh7 cells containing (WT) or lacking (DDX6 KO) DDX6. Knock out and genetic complementation by DDX6 add back was described previously [42].