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. 2019 May 22;15(5):e1007769. doi: 10.1371/journal.ppat.1007769

Fig 2. C4-1 cells are dependent on continued expression of E6 and E7 for growth and proliferation.

Fig 2

(A) Micrograph of the cells after 72h of siRNA treatment. C4-1 cells were transfected with si-Luciferase (si-Luci), si-HPV18 E6, si-HPV18E6 and E6/7 together, using RNAimax transfection reagent. (B) After 72h incubation, cells were counted to assess the remaining cell number and the graph shows the quantification after the different siRNA transfections. Asterisks indicate the p-value (*—p<0.01, ***—p<0.0003) of mean cell number in siRNA transfections compared with siLuci, using an unpaired t-test. (C) Western blots showing levels of pRB, p53, α-tubulin, 18E7 and 18E6 after transfection with the indicated siRNAs.