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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Osteoarthritis Cartilage. 2018 Dec 24;27(4):703–711. doi: 10.1016/j.joca.2018.12.010

Fig. 2.

Fig. 2.

Effect of menadione on IGF-1 signaling in human femur and talar chondrocytes. Confluent human articular chondrocytes isolated from the femur and talus of the same donor were treated for 30 min with IGF-1 (50 ng/ml) or pre-treated with menadione (25 μM) for 30 min prior to IGF-1 treatment (30 min). (A) Representative immunoblots from n = 6 independent donors showing the phosphorylation of Akt and its downstream marker of activation, PRAS40. (B-C) Densitometric analysis showing phosphorylation of Akt and pPRAS40. Phosphorylated bands were normalized to total protein as loading controls and presented as mean values ± 95% CI (n = 6).