Fig. 3.

Hydroxyproline content of skin and lungs from male C57BL6/J and Nox4 knockout mice implanted with subcutaneous osmotic pumps containing either saline or TGF-β1. Hydroxyproline content of skin (a) and lung (b) tissues isolated from male control mice treated with either saline or with TGF-β1 and from Nox4 knockout mice receiving the same treatments. (N = 5 for saline-treated C57BL6/J mice and N = 2 for saline-treated Nox4 knockout mice of each strain; N = 4 for TGF-β1-treated groups.) Samples were hydrolyzed and analyzed for hydroxyproline content (expressed as μg/mg of tissue wet weight) in triplicate. Significance determined by Student’s two-tailed t-test. ***p < 0.001