Fig. 8.

Immunohistology of oxidative stress-induced DNA damage in the lungs of male saline-treate and TGF-β1-treated C57BL6/J and Nox4 knockout mice. Confocal microscopy staining for 8-hydroxydeoxy guanosine (green) in the lungs from male saline-treated C57BL6/J (a) or Nox4 knockout mice (c), or TGF-β-treated C57BL6/J (b) or Nox4 knockout mice (d). Magnification: ×20. Saline-treated male C57BL6/J (a) and Nox4 knockout animals show little or no staining for 8-oxo-dG (green) indicative of low levels of oxidative stress-induced DNA damage. Treatment with TGF-β dramatically increases cells positive for 8-oxo-dG in C57BL6/J mice (c) whereas no comparable increase is detected in the Nox4 knockout mouse lung (d)