Fig. 2.

Functional consequences of rs146534657:A>G and rs2230926:T>G. (A) Wild-type A20 plasmid or plasmids containing the rs146534657:A>G (N102S) and rs2230926:T>G (F127C) were transfected into A20 knockout 293 cells at the indicated concentrations along with an NF-kB firefly luciferase reporter. Cells were stimulated with 10 ng/ml of TNFα (TNF) for 8 h then harvested for luciferase assay. Firefly luciferase activity was normalized to a co-transfected Renilla luciferase reporter. Total plasmid concentrations were normalized using empty pCMV vector. (B) Protein from these cells was also used in Western blot. Quantifications of transfected A20 band intensities shown as immuno-blots (IB) are normalized to the housekeeping protein GAPDH.

Note: ** P < 0.01. Figures are representative of three independent experiments.