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. Author manuscript; available in PMC: 2019 May 22.
Published in final edited form as: Nat Protoc. 2019 Feb;14(2):518–540. doi: 10.1038/s41596-018-0104-8

Fig. 1 |. Schematic of protocol and timeline.

Fig. 1 |

hPSCs in a monolayer are directed to endoderm and then anterior foregut endoderm over the course of 9–10 d. Foregut spheroids self-aggregate and lift away from the monolayer to float in the media. These spheroids are then cultured in a 3D Matrigel droplet, where they can be directed to become airway-like human lung organoids or bud tip progenitor organoids.