Table 2 |.

Media components

Media	Definitive endoderm, day 1	Definitive endoderm, day 2	Definitive endoderm, day 3	Definitive endoderm, day 4	Anterior foregut endoderm, days 5–9	Human lung organoid maintenance	Bud tip progenitor organoid medium
Basal media	RPMI 1640	RPMI 1640 0.2% (vol/vol) HyClone FBS	RPMI 1640 2% (vol/vol) HyClone FBS	RPMI 1640 2% (vol/vol) HyClone FBS	(Foregut basal) Advanced DMEM/F12, 1× N-2, 1× B27, 10 mM HEPES buffer, 1× L-glutamine (2 mM), 1× penicillin–streptomycin (5,000 U/mL)	(Foregut basal) Advanced DMEM/F12, 1× N-2, 1× B27, 10 mM HEPES buffer, 1× L-glutamine (2 mM), 1× penicillin–streptomycin (5,000 U/mL)	(Bud tip basal) DMEM F12, 1× N-2, 1× B27, 1× L-glutamine (200 mM), 1× penicillin–streptomycin (5,000 U/mL), 0.05% (vol/vol) BSA
Add on day of use	Activin A, 100 ng/mL	Activin A, 100 ng/mL	Activin A, 100 ng/mL	Activin A, 100 ng/mL	10 µM SB431542, 200 ng/mL Noggin, 1 µM SAG, 500 ng/mL FGF4, 2 µM CHIR-99021	500 ng/mL FGF10, 1% (vol/vol) FBS	0.4 µM monothio-glycerol, 50 µg/mL ascorbic acid, 10 ng/mL FGF7, 50 nM ATRA, 3 µM CHIR-99021