Figure 2.

In vitro MTN magnetofection on hMSCs and cytotoxicity. (A) The transgene expression and (B) cytotoxicity mediated by HA-SPIO incorporated with different polyplex or lipoplex were analyzed by luciferase assay and alamar blue assay, respectively. Data are mean±s.e.m. and were measured from three independent experiments. *P<0.01 by two-way ANOVA with Tukey's multiple comparisons test. The definition of abbreviations is concluded as followed. M-: without magnetic attraction. M+: with magnetic attraction. Nitrogen to Phosphate (N/P) ratio: the ratio of the amount of nitrogen in PAE or PEI to the amount of phosphate in pDNA. Volume to weight (v/w) ratio: the ratio of volume (μL) of commercial lipofectamine to the weight (μg) of pDNA.