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. 2019 Apr 13;9(8):2411–2423. doi: 10.7150/thno.29326

Figure 4.

Figure 4

Characterization of TRAILhMSCs and in vitro anti-cancer effect against glioma. The amount of TRAIL from TRAILhMSCs and its antineoplastic effect in vitro were examined by (A) ELISA assay and (B) co-culture test respectively. (C) The intracellular active caspase-3 expression of U87MG was stained by caspase-3 immunofluorescence staining. (D) The percentage of active caspase-3 positive cells were calculated by ImageJ. Data in (D) were measured from five independent experiments. In A, *P<0.01 by student's t-test. In (B) and (D), *P<0.01 by two-way ANOVA with Tukey's multiple comparisons test.