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. 2019 Apr 13;9(8):2411–2423. doi: 10.7150/thno.29326

Figure 5.

Figure 5

Evaluation of hMSCs tumor migration and penetration ability on human glioma cells. (A) The transwell system was used to evaluate the tumor tropism ability of hMSCs. For investigating penetrating ability of hMSCs, eGFP293T, eGFPhMSCs or MTN/mOrangehMSCs were incubated with U87MG spheroids for 24 hours before cryosectioning. (B) Serial cryosectioning images from top to the bottom of spheroid and the cell nucleus on these sections were stained with Hoechst 33342. Green fluorescent eGFP293T or eGFPhMSCs or red fluorescent MTN/mOrangehMSCs were observed using a fluorescence microscopy. Scale bar in (B): 100 μm. Data are mean±s.e.m. Data in (A) were measured from three independent experiments. In (A), *P<0.01 by two-way ANOVA with Tukey's multiple comparisons test.