Skip to main content
. 2019 Apr 12;9(8):2252–2267. doi: 10.7150/thno.30658

Figure 2.

Figure 2

LMNA G609G/G609G mice show accelerated IDD. (A) Representative X-ray images and disc height indices (DHI) of the IVD of WT and G609G/G609G mice; n = 3; *P < 0.05, **P < 0.01. DHI% was calculated as: DHI% = (D1+D2+D3) × 100% / (V1+V2+V3+D1+D2+D3), where D indicates disc height and V indicates vertebra length. (B) HE and safranin O staining and histological scores of the IVDs of WT and G609G/G609G mice; n = 3; **P < 0.01. (C) Representative IF images of progerin, collagen II, and aggrecan expression and TUNEL staining of IVDs in WT and G609G/G609G mice. Nuclei were stained with DAPI. (D) Representative Western blots of collagen II, aggrecan, and MMP-13 in the IVDs of WT and G609G/G609G mice; n = 3; *P < 0.05, **P < 0.01. Data represent mean ± SEM. TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick‑end labelling; MMP, matrix metalloproteinase.